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The presence of measurable residual disease (MRD) in patients with acute myeloid leukemia (AML) following intensive chemotherapy is associated with poor outcomes.1 NPM1 MRD detected by real-time quantitative polymerase chain reaction (RT-qPCR) following two courses of induction is a robust prognostic marker.2 Similarly, MRD has prognostic utility in the pretransplant setting.3,4,5,6 Patients MRD status postinduction chemotherapy may be useful to guide treatment decisions.1,4
During the 65th American Society of Hematology (ASH) Annual Meeting and Exposition, several presentations discussed the prognostic utility of MRD in patients with AML, and how this can influence treatment decisions. Below we summarize presentations from Russel.1, Othman.2,4, Loo.3, and Gui.5,6 discussing the role of MRD in treatment decisions.
The AML Hub previously reported the study design of the UK AML18 (NCT02272478). Following the first course of induction chemotherapy, eligible patients included those who had:
For course 2 of the induction therapy, patients were randomized to either continue treatment with standard daunorubicin + cytarabine (DA) or receive up to two courses of intensified chemotherapy with either fludarabine, cytarabine, granulocyte-colony stimulating factor, and idarubicin (FLAG-Ida) or DA with cladribine (DAC). In total, 523 patients were randomized (193 to DA, 191 to FLAG-Ida, and 139 to DAC). The median age was 67 years, and patient characteristics were similar between treatment arms.
Table 1. Count recovery time and supportive care requirements by treatment type in the AML18 trial*
|
DA |
FLAG-Ida |
DAC |
p-value |
---|---|---|---|---|
Blood transfusions, median units |
5 |
8 |
7 |
< 0.001 |
Platelet transfusion, median units |
4 |
8 |
7 |
< 0.001 |
Days to ANC > 1.0 × 109/L, median |
25 |
30 |
29 |
< 0.001 |
Days to platelets > 100 × 109/L, median |
26 |
34 |
33 |
< 0.001 |
6 |
13 |
11.5 |
< 0.001 |
|
Nights in hospital |
24 |
32 |
29 |
< 0.001 |
ANC, absolute neutrophil count; DA, daunorubicin + cytarabine; DAC, DA with cladribine; FLAG-Ida, fludarabine, cytarabine, granulocyte-colony stimulating factor, and idarubicin; IV, intravenously. *Adapted from Russel.1 |
Figure 1. 5-year OS rate by treatment intensification in the AML18 trial*
DA, daunorubicin + cytarabine; DAC, DA with cladribine; FLAG-Ida, fludarabine, cytarabine, granulocyte-colony stimulating factor, and idarubicin; MRD, measurable residual disease; OS, overall survival.
*Adapted from Russel.1
Figure 2. 5-year OS rate by treatment intensification in MRD+ patients censoring patients at allo-HSCT in the AML18 trial*
Allo-HSCT, allogeneic hematopoietic stem cell transplantation; DA, daunorubicin + cytarabine; DAC, DA with cladribine; FLAG-Ida, fludarabine, cytarabine, granulocyte-colony stimulating factor, and idarubicin; MRD, measurable residual disease; OS, overall survival.
*Adapted from Russel.1
Treatment intensification is associated with a survival benefit in patients with MRD+ status. DAC was better tolerated than FLAG-Ida. The survival benefit of DAC was maintained in patients who did not receive allo-HSCT as well as those who were aged >70 years, while patients treated with FLAG-Ida did not achieve long-term survival benefit.
This was a pooled analysis of data from patients with NPM1-mutated AML in the UK AML17 (ISRCTN55675535) and AML19 (ISRCTN78449203) trials. In total, 1357 patients with NPM1 mutated AML were included (AML17, n = 888; AML19, n = 469). The median follow-up was 5.15 years.
Presenter’s conclusion
In patients who achieved MRD−, DNMT3A and WT1 mutations remained associated with poor outcomes, while FLT3-ITD mutations were no longer associated with poor survival outcomes. Survival was improved in patients who received FLAG-Ida, including high-risk patients.
Pre-allo-HSCT cDNA from 66 patients with KMT2A rearranged (KMT2Ar) AML was assessed using RT-qPCR (n = 57) or RT-digital PCR (n = 9). The median age was 42 years, and the median follow-up was 37 months.
Table 2. Distribution of KMT2Ar fusion partners*
KMT2Ar fusion partner, % |
All patients (n = 66) |
Pretransplant KMT2Ar MRD status |
|
---|---|---|---|
MRD+ (n = 27) |
MRD− (n = 39) |
||
t(9;11)/KMT2A::MLLT3 |
35 |
33 |
36 |
t(6;11)/KMT2A::AFDN |
26 |
30 |
23 |
t(11;19)/KMT2 A::ELL |
17 |
22 |
13 |
t(11;19)/KMT2A::MLLT1 |
5 |
11 |
0 |
t(10;11)/KMT2A::MLLT10 |
15 |
4 |
23 |
Other |
3 |
0 |
5 |
KMT2Ar, KMT2A rearranged; MRD, measurable residual disease; t, translocation. |
Figure 3. 2-year RFS, OS, and CIR rate by KMT2Ar MRD status*
CIR cumulative incidence of relapse; KMT2Ar, KMT2A rearranged; MRD, measurable residual disease; OS, overall survival; RFS, relapse-free survival.
*Data from Loo.3
KMT2Ar MRD detected by RT-qPCR/RT-digital PCR pretransplant is associated with worse posttransplant survival outcomes.
This analysis assessed the impact of MRD status on transplant outcomes in patients with NPM1-mutated AML by combining data from the UK AML17 (ISRCTN55675535) and AML19 (ISRCTN78449203) trials. In the AML17 trial, patients with NPM1-mutated AML in CR1 were selected for transplant based on a validated risk score; whereas, in the AML19 trial, selection was based on MRD status after two courses of chemotherapy, regardless of risk factors. In total, 737 patients were included in this analysis (AML17, n = 348; AML19, n = 389).
Postinduction MRD status can be used to identify patients with NPM1-mutated AML who are likely to benefit from transplantation in CR1.
Adult patients in CR1 with IDH1- or IDH2-mutated AML undergoing allo-HSCT between 2013 and 2019 were included in these analyses as part of the Pre-MEASURE study. In both analyses, MRD was assessed from peripheral blood samples collected ≤100 days before allo-HSCT using next-generation sequencing.5,6
Table 3. Factors associated with posttransplant outcomes in the IDH2 cohort from the Pre-MEASURE study*
OS |
HR (95% CI) |
p-value |
---|---|---|
NPM1/FLT3-ITD MRD+ |
10 (3.2–31) |
<0.001 |
IDH2 MRD+ |
4.4 (1.4–13.7) |
0.01 |
Relapse |
HR (95% CI) |
p-value |
NPM1/FLT3-ITD MRD+ |
21.0 (7.0–63) |
<0.001 |
Age, >60 years |
5.0 (1.4–18) |
0.02 |
Age, every 1 year above 40 |
0.95 (0.9–0.97) |
<0.001 |
IDH1 baseline, positive |
4.7 (1.5–14.3) |
0.007 |
Antithymocyte globulin, yes |
2.9 (1.1–7.3) |
0.03 |
Sex, male |
0.3 (0.1–0.9) |
0.03 |
CI, confidence interval; HR, hazard ratio; ITD, internal tandem duplication; MRD+, measurable residual disease positive; OS, overall survival. *Adapted from Gui.6 |
Pre-allo-HSCT IDH1 MRD status was not associated with post-allo-HSCT outcomes.5 However, pre-allo-HSCT NMP1 and/or FLT3-ITD MRD positivity was associated with an increased risk of relapse in patients with IDH1-mutated and NMP1 and/or FLT3-ITD-mutated AML.5 Conversely, pre-allo-HSCT IDH2 MRD positivity was predictive of inferior post-allo-HSCT outcomes.6 In patients with IDH2 and NMP1 and/or FLT3-ITD mutations at baseline, NMP1 and/or FLT3-ITD MRD status was a superior prognostic marker than IDH2 MRD status.6
Yes, in 1-6 months
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Yes, in 6-12 months
0%
Yes, in 1-2 years
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No
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