All content on this site is intended for healthcare professionals only. By acknowledging this message and accessing the information on this website you are confirming that you are a Healthcare Professional. If you are a patient or carer, please visit Know AML.

The AML Hub uses cookies on this website. They help us give you the best online experience. By continuing to use our website without changing your cookie settings, you agree to our use of cookies in accordance with our updated Cookie Policy

Introducing

Now you can personalise
your AML Hub experience!

Bookmark content to read later

Select your specific areas of interest

View content recommended for you

Find out more
  TRANSLATE

The AML Hub website uses a third-party service provided by Google that dynamically translates web content. Translations are machine generated, so may not be an exact or complete translation, and the AML Hub cannot guarantee the accuracy of translated content. The AML Hub and its employees will not be liable for any direct, indirect, or consequential damages (even if foreseeable) resulting from use of the Google Translate feature. For further support with Google Translate, visit Google Translate Help.

Steering CommitteeAbout UsNewsletterContact
LOADING
You're logged in! Click here any time to manage your account or log out.
LOADING
You're logged in! Click here any time to manage your account or log out.
2020-08-07T16:25:16.000Z

Outcomes of two or more HLA loci mismatched unrelated donor allo-HCT: An Acute Leukemia Working Party study

Aug 7, 2020
Share:

Bookmark this article

The optimal allogeneic hematopoietic cell transplantation (allo-HCT) results depend on the availability of a human leukocyte antigen (HLA)-matched donor. Patients who lack an HLA-identical donor have limited donor options, specifically a haploidentical related donor, umbilical cord blood unit, or a mismatched unrelated donor (MMUD). High-resolution HLA allele level typing has improved the assessment of MMUD allo-HCT, and it has been found that increasing numbers of mismatched HLA loci correlate with a higher risk of non-relapse mortality (NRM) and graft-versus-host disease (GvHD; find out more here). However, the impact of two or more HLA mismatches on outcomes in transplanted patients with acute leukemia is unknown.

GvHD Hub Steering Committee member Arnon Nagler and colleagues performed a retrospective, multicenter study using the data set from the Acute Leukemia Working Party of the European Society for Blood and Marrow Transplantation (EBMT) to assess the outcome of patients with acute leukemia transplanted from ≥ 2 HLA allele MMUD in first or second complete remission (CR1 or CR2) who underwent first allo-HCT. Nagler et al. published their study in Bone Marrow Transplantation.1

Study design

The inclusion criteria for this study were defined as adult patients aged ≥ 18 years with acute leukemia (acute myelogenous leukemia [AML] or acute lymphoblastic leukemia [ALL]), transplanted from ≥ 2 HLA allele MMUD (at HLA-A, -B, -C, or -DR loci between the donor and recipient), in CR1 or CR2, who underwent first allo-HCT between 2000 and 2017.

Results

A total of 145 patients with ALL and 320 patients with AML, who met the inclusion criteria, were selected for this study and followed for a median of 74.8 months (ALL) or 63.3 months (AML). The median patient age at the time of allo-HCT was higher in patients with AML compared with ALL (50.2 years and 34.8 years, respectively), and most patients had a high Karnofsky Performance Status score. Also, the majority of patients had a peripheral blood stem cell graft, myeloablative conditioning, and received in vivo T-cell depletion. Detailed baseline patient, disease, and transplant characteristics are described in Table 1.

Table 1. Baseline patient, disease, and transplant characteristics.1

ALL, acute lymphoblastic leukemia; AML, acute myeloid leukemia; ATG, antithymocyte globulin; BM, bone marrow; CR1, first complete remission; CR2, second complete remission; CSA, cyclosporin A; GvHD, graft-versus-host disease; HLA, human leukocyte antigen; IQR, interquartile range; KPS, Karnofsky performance status; MAC, myeloablative conditioning; MMF, mycophenolate mofetil; MTX, methotrexate; PB, peripheral blood; Ph, Philadelphia chromosome; PTCy, post-transplant cyclophosphamide; RIC, reduced-intensity conditioning; tacro, tacrolimus; Tx, transplantation

* per United Kingdom Medical Research Council classification (MRC-C).

t(v;11w23) = 2 patients; t(1;19)(q23;p13.3) = 2 patients; hyperdiploidy = 1 patient.

 

 

ALL

(N = 145)

AML

(N = 320)

Patient age at Tx

Median (range) [IQR]

34.8

(18.2–62.9)

[25.3–45.9]

50.2

(18–71.8)

[40.2–59.1]

Disease status at Tx

CR1

CR2+

96 (66.2%)

49 (33.8%)

222 (69.4%)

98 (30.6%)

Secondary AML

56 (17.5%)

Cytogenetics

of AML*

Good-risk

Intermediate-risk

Poor-risk

Not available/failed

15 (4.7%)

129 (40.3%)

33 (10.3%)

143 (44.7%)

ALL subtype

Ph-negative B-ALL

Ph-positive B-ALL

Ph unknown B-ALL

T-ALL

Missing

immunophenotype

32 (22.1%)

53 (36.6%)

25 (17.2%)

26 (17.9%)

9 (6.2%)

KPS

< 90

≥ 90

Missing

26 (17.9%)

94 (64.8%)

25 (17.2%)

68 (21.3%)

216 (67.5%)

36 (11.3%)

Graft source 

BM

PB

34 (23.5%)

111 (76.6%)

48 (15%)

272 (85%)

Conditioning

regimen

MAC

RIC

Missing

129 (89.0%)

16 (11.0%)

0

192 (60%)

125 (39.1%)

3 (0.9%)

In vivo T-cell

depletion

None

ATG

Alemtuzumab

33 (22.8%)

99 (68.3%)

13 (9.0%)

58 (18.1%)

226 (70.6%)

36 (11.3%)

GvHD prophylaxis

CSA + MTX

CSA + MMF

Tacro + MTX

Tacro + MMF

CSA

PTCy ± other

Other

97 (66.9%)

15 (10.3%)

4 (2.8%)

3 (2.1%)

10 (6.9%)

4 (2.8%)

12 (8.3%)

162 (51%)

65 (20%)

13 (4.1%)

12 (3.6%)

29 (9.1%)

18 (5.6%)

21 (6.6%)

Details on HLA mismatch

 

 

Number of mismatched loci (out of 8)

2

3

4

123 (84.8%)

18 (12.4%)

4 (2.8%)

263 (82.2%)

37 (11.6%)

20 (6.3%)

Number of patients with each mismatched locus

A

B

C

DR

40

45

62

23

106

104

131

51


Using Cox-regression for multivariate analysis, the authors identified HLA-DR mismatch as a bad prognostic indicator, showing a remarkably higher NRM (HR, 1.67; p = 0.02), poorer leukemia-free survival (HR, 1.42; p = 0.03), overall survival (OS; HR, 1.46; p = 0.03), and higher acute (a)GVHD Grade 2–4 (HR, 1.46; p = 0.05). However, no impact was seen on relapse incidence, refined GvHD-free, relapse-free survival, or overall chronic (c)GvHD.  

Also, a higher risk of aGvHD Grade 3–4 (HR, 1.87; 95% CI, 1.08–3.22; p = 0.02) was seen in patients with more than two mismatched HLA loci while all other outcome parameters were not affected.

Finally, OS was negatively impacted by CR2 disease status (HR, 1.43; p = 0.01) and increasing patient age (per 10 years; HR, 1.17; p = 0.01).

Stem cell graft source (bone marrow vs peripheral blood) or type of conditioning regimen (MAC vs RIC) were not identified as independent factors for outcome.

The most common cause of death was disease relapse, occurring in 37% of patients with ALL and 37% of patients with AML. The second most common cause was related to infection (AML, 25% and ALL, 22%), followed by GvHD-related deaths (AML, 22% and ALL, 29%).

Results of 2-year and 5-year allo-HCT outcomes are presented in Table 2.

Table 2. Outcomes of mismatch unrelated donor hematopoietic cell transplantation.1

cGvHD, chronic GvHD; CI, confidence interval; GRFS, GvHD-free, relapse-free survival; GvHD, graft-versus-host disease; LFS, leukemia-free survival; NRM, non-relapse mortality; OS, overall survival

Outcome

2-year outcome, % (95% CI)

5-year outcome, % (95% CI)

Relapse incidence

28.1 (23.9–32.4)

32.9 (28.4–37.5)

NRM 

28.1 (23.9–32.3)

30.5 (26.1–34.9)

LFS

43.8 (39.1–48.5)

36.6 (31.9–41.4)

OS 

49.9 (45.1–54.7)

41.2 (36.4–46.1)

Refined GRFS

33.4 (28.9–37.8)

26.6 (22.3–30.9)

Overall cGVHD 

38.1 (33.3–42.8)

40.7 (35.7–45.5)

Extensive cGVHD 

17 (13.5–20.9)

20.7 (16.7–25.1)

Conclusion

This retrospective, multicenter study found that allo-HCT with ≤ 6 out of eight HLA allele MMUD in patients with acute leukemia led to an acceptable 2-year leukemia-free survival, and an OS which was comparable to allo-HCT from matched unrelated donors. However, mismatch at the HLA-DR locus and mismatch at more than two HLA-alleles were considered to be poor prognostic factors for the risk of NRM and aGvHD, respectively. These two factors should be considered in the donor selection process.

  1. Nagler A, Dholaria B, Labopin M, et al. The outcome of two or more HLA loci mismatched unrelated donor hematopoietic cell transplantation for acute leukemia: an ALWP of the EBMT study. Bone marrow transplantation. 2020. Online ahead of print. DOI: 1038/s41409-020-0974-6

Your opinion matters

Do you intend to implement next generation sequencing for measurable residual disease monitoring in AML patients?
0 votes - 6 days left ...

Newsletter

Subscribe to get the best content related to AML delivered to your inbox